Post-translational Processing Site of Rat Fetal Liver Histidine Decarboxylase.
نویسندگان
چکیده
منابع مشابه
Histidine decarboxylase. Purification from fetal rat liver, immunologic properties, and histochemical localization in brain and stomach.
Histidine decarboxylase from fetal rat liver was purified to near-homogeneity. The purified enzyme has a molecular weight of 210,000, and appears to contain two subunits with molecular weights of 145,000 and 66,000, respectively. The enzyme is inhibited by heavy metals such as Hg2+ and Zn2+ and sulfhydryl-reactive compounds such as 5,5'-dithiobis-2-nitrobenzoic acid. The enzyme is partially dep...
متن کاملSpectroscopic analysis of recombinant rat histidine decarboxylase.
Mammalian histidine decarboxylases have not been characterized well owing to their low amounts in tissues and instability. We describe here the first spectroscopic characterization of a mammalian histidine decarboxylase, i.e. a recombinant version of the rat enzyme purified from transformed Escherichia coli cultures, with similar kinetic constants to those reported for mammalian histidine decar...
متن کاملRat Liver Aminomalonate Decarboxylase
Studies on the aminomalonate decarboxylase of rat liver indicate that this activity is a property of cytoplasmic serine hydroxymethylase. Thus, throughout purification of the enzyme, aminomalonate decarboxylase, serine hydroxymethylase and allothreonine aldolase exhibited the same relative activities. Competition between the several substrates was observed and resolution of the enzyme by treatm...
متن کاملIn vitro study of proteolytic degradation of rat histidine decarboxylase.
Mammalian ornithine decarboxylase (ODC) is a very unstable protein which is degraded in an ATP-dependent manner by proteasome 26S, after making contact with the regulatory protein antizyme. PEST regions are sequences described as signals for protein degradation. The C-terminal PEST region of mammalian ODC is essential for its degradation by proteasome 26S. Mammalian histidine decarboxylase (HDC...
متن کاملEffect of hydrocortisone on histidine decarboxylase activity in rat stomach.
Hydrocortisone-stimulated synthesis of histidine decarboxylase (EC 4.1.1.22) was studied in rat stomach. Single or daily administration of hydrocortisone increased the histidine decarboxylase activity but not the histamine content in the fundic glands of stomachs from fed and fasted rats with or without adrenalectomy, and also in those from adrenalectomized fasted rats with concomitant treatmen...
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ژورنال
عنوان ژورنال: Japanese Journal of Pharmacology
سال: 1991
ISSN: 0021-5198
DOI: 10.1016/s0021-5198(19)39490-9